Construct: shRNA TRCN0000003490

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000063.x-1476s1c1
DNA Barcode:
GCATGTCACTATTAAGCCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C2 (717)
Gene Description:
complement C2
Transcript:
RefSeq NM_000063.3 (NON-CURRENT)
Match location:
Position 1476 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003490)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTCACTATTAAGCCCAA
Hairpin Sequence:
5'-CCGG-GCATGTCACTATTAAGCCCAA-CTCGAG-TTGGGCTTAATAGTGACATGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTCACTATTAAGCCCAACTCGAGTTGGGCTTAATAGTGACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTCACTATTAAGCCCAACTCGAGTTGGGCTTAATAGTGACATGC-3'

Other clones with same target sequence:

(none)