Construct: shRNA TRCN0000003495

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000070.x-3063s1c1
DNA Barcode:
GCTCCTGCTTACCTTGCTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAPN3 (825)
Gene Description:
calpain 3
Transcript:
RefSeq NM_000070.2 (NON-CURRENT)
Match location:
Position 3063 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003495)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCTGCTTACCTTGCTCTA
Hairpin Sequence:
5'-CCGG-GCTCCTGCTTACCTTGCTCTA-CTCGAG-TAGAGCAAGGTAAGCAGGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCTGCTTACCTTGCTCTACTCGAGTAGAGCAAGGTAAGCAGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCTGCTTACCTTGCTCTACTCGAGTAGAGCAAGGTAAGCAGGAGC-3'

Other clones with same target sequence:

(none)