Construct: shRNA TRCN0000003547
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001749.x-871s1c1
- DNA Barcode:
- CCATGTTCCGTGCCTTCAAAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAPNS1 (826)
- Gene Description:
- calpain small subunit 1
- Transcript:
- RefSeq NM_001749.1 (NON-CURRENT)
- Match location:
- Position 871 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000003547)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCATGTTCCGTGCCTTCAAAT
- Hairpin Sequence:
-
5'-CCGG-CCATGTTCCGTGCCTTCAAAT-CTCGAG-ATTTGAAGGCACGGAACATGG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCATGTTCCGTGCCTTCAAATCTCGAGATTTGAAGGCACGGAACATGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCATGTTCCGTGCCTTCAAATCTCGAGATTTGAAGGCACGGAACATGG-3'
Other clones with same target sequence:
-
TRCN0000318537
