Construct: shRNA TRCN0000003574

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000391.x-1000s1c1
DNA Barcode:
GCTATGGAGATGATGAGGACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPP1 (1200)
Gene Description:
tripeptidyl peptidase 1
Transcript:
RefSeq NM_000391.2 (NON-CURRENT)
Match location:
Position 1000 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003574)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATGGAGATGATGAGGACT
Hairpin Sequence:
5'-CCGG-GCTATGGAGATGATGAGGACT-CTCGAG-AGTCCTCATCATCTCCATAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATGGAGATGATGAGGACTCTCGAGAGTCCTCATCATCTCCATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATGGAGATGATGAGGACTCTCGAGAGTCCTCATCATCTCCATAGC-3'

Other clones with same target sequence:

(none)