Construct: shRNA TRCN0000003597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001242.x-1250s1c1
DNA Barcode:
CGAAAGACCCACATGCTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CD27 (939)
Gene Description:
CD27 molecule
Transcript:
RefSeq NM_001242.3 (NON-CURRENT)
Match location:
Position 1250 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAAGACCCACATGCTACAA
Hairpin Sequence:
5'-CCGG-CGAAAGACCCACATGCTACAA-CTCGAG-TTGTAGCATGTGGGTCTTTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAAGACCCACATGCTACAACTCGAGTTGTAGCATGTGGGTCTTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAAGACCCACATGCTACAACTCGAGTTGTAGCATGTGGGTCTTTCG-3'

Other clones with same target sequence:

(none)