Construct: shRNA TRCN0000003680

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001971.x-401s1c1
DNA Barcode:
CGTTACCCTCAATAGCTATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CELA1 (1990)
Gene Description:
chymotrypsin like elastase 1
Transcript:
RefSeq NM_001971.3 (NON-CURRENT)
Match location:
Position 401 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003680)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTACCCTCAATAGCTATGT
Hairpin Sequence:
5'-CCGG-CGTTACCCTCAATAGCTATGT-CTCGAG-ACATAGCTATTGAGGGTAACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTACCCTCAATAGCTATGTCTCGAGACATAGCTATTGAGGGTAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTACCCTCAATAGCTATGTCTCGAGACATAGCTATTGAGGGTAACG-3'

Other clones with same target sequence:

(none)