Construct: shRNA TRCN0000003753

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000546.x-1095s1c1
DNA Barcode:
CGGCGCACAGAGGAAGAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TP53 (7157)
Gene Description:
tumor protein p53
Transcript:
RefSeq NM_000546.2 (NON-CURRENT)
Match location:
Position 1095 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003753)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCGCACAGAGGAAGAGAAT
Hairpin Sequence:
5'-CCGG-CGGCGCACAGAGGAAGAGAAT-CTCGAG-ATTCTCTTCCTCTGTGCGCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCGCACAGAGGAAGAGAATCTCGAGATTCTCTTCCTCTGTGCGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCGCACAGAGGAAGAGAATCTCGAGATTCTCTTCCTCTGTGCGCCG-3'

Other clones with same target sequence:

  1. TRCN0000342335