Construct: shRNA TRCN0000003806

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001430.x-1694s1c1
DNA Barcode:
CAGTACCCAGACGGATTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPAS1 (2034)
Gene Description:
endothelial PAS domain protein 1
Transcript:
RefSeq NM_001430.1 (NON-CURRENT)
Match location:
Position 1694 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003806)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTACCCAGACGGATTTCAA
Hairpin Sequence:
5'-CCGG-CAGTACCCAGACGGATTTCAA-CTCGAG-TTGAAATCCGTCTGGGTACTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTACCCAGACGGATTTCAACTCGAGTTGAAATCCGTCTGGGTACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTACCCAGACGGATTTCAACTCGAGTTGAAATCCGTCTGGGTACTG-3'

Other clones with same target sequence:

  1. TRCN0000342501