Construct: shRNA TRCN0000003853

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001987.x-1218s1c1
DNA Barcode:
GCGCCACTACTACAAACTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ETV6 (2120)
Gene Description:
ETS variant transcription factor 6
Transcript:
RefSeq NM_001987.2 (NON-CURRENT)
Match location:
Position 1218 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003853)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCCACTACTACAAACTAAA
Hairpin Sequence:
5'-CCGG-GCGCCACTACTACAAACTAAA-CTCGAG-TTTAGTTTGTAGTAGTGGCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCCACTACTACAAACTAAACTCGAGTTTAGTTTGTAGTAGTGGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCCACTACTACAAACTAAACTCGAGTTTAGTTTGTAGTAGTGGCGC-3'

Other clones with same target sequence:

  1. TRCN0000338363