Construct: shRNA TRCN0000003854

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001987.x-1677s1c1
DNA Barcode:
GTTGTTAGTATCATGGTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ETV6 (2120)
Gene Description:
ETS variant transcription factor 6
Transcript:
RefSeq NM_001987.2 (NON-CURRENT)
Match location:
Position 1677 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003854)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGTTAGTATCATGGTGTTT
Hairpin Sequence:
5'-CCGG-GTTGTTAGTATCATGGTGTTT-CTCGAG-AAACACCATGATACTAACAAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGTTAGTATCATGGTGTTTCTCGAGAAACACCATGATACTAACAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGTTAGTATCATGGTGTTTCTCGAGAAACACCATGATACTAACAAC-3'

Other clones with same target sequence:

  1. TRCN0000338364