Construct: shRNA TRCN0000003902

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002793.x-499s1c1
DNA Barcode:
GCTTTGATCCAGTAGGGTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSMB1 (5689)
Gene Description:
proteasome 20S subunit beta 1
Transcript:
RefSeq NM_002793.2 (NON-CURRENT)
Match location:
Position 499 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003902)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGATCCAGTAGGGTCTT
Hairpin Sequence:
5'-CCGG-GCTTTGATCCAGTAGGGTCTT-CTCGAG-AAGACCCTACTGGATCAAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGATCCAGTAGGGTCTTCTCGAGAAGACCCTACTGGATCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGATCCAGTAGGGTCTTCTCGAGAAGACCCTACTGGATCAAAGC-3'

Other clones with same target sequence:

  1. TRCN0000277847