Construct: shRNA TRCN0000003997

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003333.x-401s1c1
DNA Barcode:
GCGTCCCAAGAAGAAGGTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBA52 (7311)
Gene Description:
ubiquitin A-52 residue ribosomal protein fusion product 1
Transcript:
RefSeq NM_003333.2 (NON-CURRENT)
Match location:
Position 401 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003997)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTCCCAAGAAGAAGGTCAA
Hairpin Sequence:
5'-CCGG-GCGTCCCAAGAAGAAGGTCAA-CTCGAG-TTGACCTTCTTCTTGGGACGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTCCCAAGAAGAAGGTCAACTCGAGTTGACCTTCTTCTTGGGACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTCCCAAGAAGAAGGTCAACTCGAGTTGACCTTCTTCTTGGGACGC-3'

Other clones with same target sequence:

  1. TRCN0000272708