Construct: shRNA TRCN0000004016

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003341.x-618s1c1
DNA Barcode:
CCTCCTTTCTATCTGCTCACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBE2E1 (7324)
Gene Description:
ubiquitin conjugating enzyme E2 E1
Transcript:
RefSeq NM_003341.3 (NON-CURRENT)
Match location:
Position 618 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004016)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCTTTCTATCTGCTCACT
Hairpin Sequence:
5'-CCGG-CCTCCTTTCTATCTGCTCACT-CTCGAG-AGTGAGCAGATAGAAAGGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCTTTCTATCTGCTCACTCTCGAGAGTGAGCAGATAGAAAGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCTTTCTATCTGCTCACTCTCGAGAGTGAGCAGATAGAAAGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000279827