Construct: shRNA TRCN0000004027

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003344.x-555s1c1
DNA Barcode:
GAGTGGACCTACCTGATAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBE2H (7328)
Gene Description:
ubiquitin conjugating enzyme E2 H
Transcript:
RefSeq NM_003344.2 (NON-CURRENT)
Match location:
Position 555 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004027)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGGACCTACCTGATAAAT
Hairpin Sequence:
5'-CCGG-GAGTGGACCTACCTGATAAAT-CTCGAG-ATTTATCAGGTAGGTCCACTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGGACCTACCTGATAAATCTCGAGATTTATCAGGTAGGTCCACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGGACCTACCTGATAAATCTCGAGATTTATCAGGTAGGTCCACTC-3'

Other clones with same target sequence:

(none)