Construct: shRNA TRCN0000004031

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003349.x-292s1c1
DNA Barcode:
ACCTACTTCTGCATCACCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM189-UBE2V1 (387522)
Gene Description:
TMEM189-UBE2V1 readthrough
Transcript:
RefSeq NM_003349.4 (NON-CURRENT)
Match location:
Position 292 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004031)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTACTTCTGCATCACCACA
Hairpin Sequence:
5'-CCGG-ACCTACTTCTGCATCACCACA-CTCGAG-TGTGGTGATGCAGAAGTAGGT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTACTTCTGCATCACCACACTCGAGTGTGGTGATGCAGAAGTAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTACTTCTGCATCACCACACTCGAGTGTGGTGATGCAGAAGTAGGT-3'

Other clones with same target sequence:

(none)