Construct: shRNA TRCN0000004036

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003350.x-480s1c1
DNA Barcode:
GTCTTAAATCAACAACCTTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBE2V2 (7336)
Gene Description:
ubiquitin conjugating enzyme E2 V2
Transcript:
RefSeq NM_003350.2 (NON-CURRENT)
Match location:
Position 480 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004036)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTTAAATCAACAACCTTCT
Hairpin Sequence:
5'-CCGG-GTCTTAAATCAACAACCTTCT-CTCGAG-AGAAGGTTGTTGATTTAAGAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTTAAATCAACAACCTTCTCTCGAGAGAAGGTTGTTGATTTAAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTTAAATCAACAACCTTCTCTCGAGAGAAGGTTGTTGATTTAAGAC-3'

Other clones with same target sequence:

  1. TRCN0000273328