Construct: shRNA TRCN0000004039

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003363.x-973s1c1
DNA Barcode:
GCACCACTGACTGACTACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
USP4 (7375)
Gene Description:
ubiquitin specific peptidase 4
Transcript:
RefSeq NM_003363.1 (NON-CURRENT)
Match location:
Position 973 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004039)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCACTGACTGACTACTTT
Hairpin Sequence:
5'-CCGG-GCACCACTGACTGACTACTTT-CTCGAG-AAAGTAGTCAGTCAGTGGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCACTGACTGACTACTTTCTCGAGAAAGTAGTCAGTCAGTGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCACTGACTGACTACTTTCTCGAGAAAGTAGTCAGTCAGTGGTGC-3'

Other clones with same target sequence:

  1. TRCN0000320389