Construct: shRNA TRCN0000004049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003449.x-1122s1c1
DNA Barcode:
GCTGAGAGACTTGGAATATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM26 (7726)
Gene Description:
tripartite motif containing 26
Transcript:
RefSeq NM_003449.2 (NON-CURRENT)
Match location:
Position 1122 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGAGACTTGGAATATAA
Hairpin Sequence:
5'-CCGG-GCTGAGAGACTTGGAATATAA-CTCGAG-TTATATTCCAAGTCTCTCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGAGACTTGGAATATAACTCGAGTTATATTCCAAGTCTCTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGAGACTTGGAATATAACTCGAGTTATATTCCAAGTCTCTCAGC-3'

Other clones with same target sequence:

(none)