Construct: shRNA TRCN0000004065

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003473.x-1869s1c1
DNA Barcode:
CTGCTAAATGCCACTGACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAM (8027)
Gene Description:
signal transducing adaptor molecule
Transcript:
RefSeq NM_003473.2 (NON-CURRENT)
Match location:
Position 1869 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004065)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCTAAATGCCACTGACAAT
Hairpin Sequence:
5'-CCGG-CTGCTAAATGCCACTGACAAT-CTCGAG-ATTGTCAGTGGCATTTAGCAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCTAAATGCCACTGACAATCTCGAGATTGTCAGTGGCATTTAGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCTAAATGCCACTGACAATCTCGAGATTGTCAGTGGCATTTAGCAG-3'

Other clones with same target sequence:

(none)