Construct: shRNA TRCN0000004073

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003603.x-2246s1c1
DNA Barcode:
CGGAGAAATCGGAGAAGCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SORBS2 (8470)
Gene Description:
sorbin and SH3 domain containing 2
Transcript:
RefSeq NM_003603.3 (NON-CURRENT)
Match location:
Position 2246 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004073)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGAAATCGGAGAAGCTAT
Hairpin Sequence:
5'-CCGG-CGGAGAAATCGGAGAAGCTAT-CTCGAG-ATAGCTTCTCCGATTTCTCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGAAATCGGAGAAGCTATCTCGAGATAGCTTCTCCGATTTCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGAAATCGGAGAAGCTATCTCGAGATAGCTTCTCCGATTTCTCCG-3'

Other clones with same target sequence:

(none)