Construct: shRNA TRCN0000004091

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015436.x-691s1c1
DNA Barcode:
GCAATGACTGTAATGGACGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RCHY1 (25898)
Gene Description:
ring finger and CHY zinc finger domain containing 1
Transcript:
RefSeq NM_015436.1 (NON-CURRENT)
Match location:
Position 691 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004091)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATGACTGTAATGGACGAT
Hairpin Sequence:
5'-CCGG-GCAATGACTGTAATGGACGAT-CTCGAG-ATCGTCCATTACAGTCATTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATGACTGTAATGGACGATCTCGAGATCGTCCATTACAGTCATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATGACTGTAATGGACGATCTCGAGATCGTCCATTACAGTCATTGC-3'

Other clones with same target sequence:

(none)