Construct: shRNA TRCN0000004147

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016166.x-933s1c1
DNA Barcode:
CATGGCAGTATATCTTGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIAS1 (8554)
Gene Description:
protein inhibitor of activated STAT 1
Transcript:
RefSeq NM_016166.1 (NON-CURRENT)
Match location:
Position 933 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004147)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGGCAGTATATCTTGTAAA
Hairpin Sequence:
5'-CCGG-CATGGCAGTATATCTTGTAAA-CTCGAG-TTTACAAGATATACTGCCATG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGGCAGTATATCTTGTAAACTCGAGTTTACAAGATATACTGCCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGGCAGTATATCTTGTAAACTCGAGTTTACAAGATATACTGCCATG-3'

Other clones with same target sequence:

(none)