Construct: shRNA TRCN0000004208
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_017610.x-4158s1c1
- DNA Barcode:
- GCTCTGATGAACACTAAATAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RNF111 (54778)
- Gene Description:
- ring finger protein 111
- Transcript:
- RefSeq NM_017610.6 (NON-CURRENT)
- Match location:
- Position 4158 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000004208)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTCTGATGAACACTAAATAT
- Hairpin Sequence:
-
5'-CCGG-GCTCTGATGAACACTAAATAT-CTCGAG-ATATTTAGTGTTCATCAGAGC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTCTGATGAACACTAAATATCTCGAGATATTTAGTGTTCATCAGAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTCTGATGAACACTAAATATCTCGAGATATTTAGTGTTCATCAGAGC-3'
Other clones with same target sequence:
(none)
