Construct: shRNA TRCN0000004239

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006986.x-2455s1c1
DNA Barcode:
CATTGGTTTCTTCTGGGTTGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGED1 (9500)
Gene Description:
MAGE family member D1
Transcript:
RefSeq NM_006986.2 (NON-CURRENT)
Match location:
Position 2455 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004239)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTGGTTTCTTCTGGGTTGA
Hairpin Sequence:
5'-CCGG-CATTGGTTTCTTCTGGGTTGA-CTCGAG-TCAACCCAGAAGAAACCAATG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTGGTTTCTTCTGGGTTGACTCGAGTCAACCCAGAAGAAACCAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTGGTTTCTTCTGGGTTGACTCGAGTCAACCCAGAAGAAACCAATG-3'

Other clones with same target sequence:

  1. TRCN0000277723