Construct: shRNA TRCN0000004247

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007051.x-2491s1c1
DNA Barcode:
CGCTCGTCTCACTCAATTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAF1 (11124)
Gene Description:
Fas associated factor 1
Transcript:
RefSeq NM_007051.2 (NON-CURRENT)
Match location:
Position 2491 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004247)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTCGTCTCACTCAATTCAA
Hairpin Sequence:
5'-CCGG-CGCTCGTCTCACTCAATTCAA-CTCGAG-TTGAATTGAGTGAGACGAGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTCGTCTCACTCAATTCAACTCGAGTTGAATTGAGTGAGACGAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTCGTCTCACTCAATTCAACTCGAGTTGAATTGAGTGAGACGAGCG-3'

Other clones with same target sequence:

  1. TRCN0000318799