Construct: shRNA TRCN0000004265

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007315.x-2339s1c1
DNA Barcode:
CCCTGAAGTATCTGTATCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAT1 (6772)
Gene Description:
signal transducer and activator of transcription 1
Transcript:
RefSeq NM_007315.2 (NON-CURRENT)
Match location:
Position 2339 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004265)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGAAGTATCTGTATCCAA
Hairpin Sequence:
5'-CCGG-CCCTGAAGTATCTGTATCCAA-CTCGAG-TTGGATACAGATACTTCAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGAAGTATCTGTATCCAACTCGAGTTGGATACAGATACTTCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGAAGTATCTGTATCCAACTCGAGTTGGATACAGATACTTCAGGG-3'

Other clones with same target sequence:

  1. TRCN0000280024