Construct: shRNA TRCN0000004294

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012161.x-697s1c1
DNA Barcode:
GTCAGAACACTCCACAGGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXL5 (26234)
Gene Description:
F-box and leucine rich repeat protein 5
Transcript:
RefSeq NM_012161.2 (NON-CURRENT)
Match location:
Position 697 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004294)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAGAACACTCCACAGGTAT
Hairpin Sequence:
5'-CCGG-GTCAGAACACTCCACAGGTAT-CTCGAG-ATACCTGTGGAGTGTTCTGAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAGAACACTCCACAGGTATCTCGAGATACCTGTGGAGTGTTCTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAGAACACTCCACAGGTATCTCGAGATACCTGTGGAGTGTTCTGAC-3'

Other clones with same target sequence:

(none)