Construct: shRNA TRCN0000004381

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014160.x-1263s1c1
DNA Barcode:
GACCTCTTCATGCACCTTTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MKRN2 (23609)
Gene Description:
makorin ring finger protein 2
Transcript:
RefSeq NM_014160.3 (NON-CURRENT)
Match location:
Position 1263 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004381)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTCTTCATGCACCTTTCT
Hairpin Sequence:
5'-CCGG-GACCTCTTCATGCACCTTTCT-CTCGAG-AGAAAGGTGCATGAAGAGGTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTCTTCATGCACCTTTCTCTCGAGAGAAAGGTGCATGAAGAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTCTTCATGCACCTTTCTCTCGAGAGAAAGGTGCATGAAGAGGTC-3'

Other clones with same target sequence:

(none)