Construct: shRNA TRCN0000004543

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020654.x-3681s1c1
DNA Barcode:
AGTGCAGCTTATTATTCTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SENP7 (57337)
Gene Description:
SUMO specific peptidase 7
Transcript:
RefSeq NM_020654.2 (NON-CURRENT)
Match location:
Position 3681 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004543)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGCAGCTTATTATTCTCAA
Hairpin Sequence:
5'-CCGG-AGTGCAGCTTATTATTCTCAA-CTCGAG-TTGAGAATAATAAGCTGCACT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGCAGCTTATTATTCTCAACTCGAGTTGAGAATAATAAGCTGCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGCAGCTTATTATTCTCAACTCGAGTTGAGAATAATAAGCTGCACT-3'

Other clones with same target sequence:

(none)