Construct: shRNA TRCN0000004551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020718.x-1591s1c1
DNA Barcode:
CTCTGCCTGATGTGCTTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
USP31 (57478)
Gene Description:
ubiquitin specific peptidase 31
Transcript:
RefSeq NM_020718.1 (NON-CURRENT)
Match location:
Position 1591 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTGCCTGATGTGCTTATTA
Hairpin Sequence:
5'-CCGG-CTCTGCCTGATGTGCTTATTA-CTCGAG-TAATAAGCACATCAGGCAGAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTGCCTGATGTGCTTATTACTCGAGTAATAAGCACATCAGGCAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTGCCTGATGTGCTTATTACTCGAGTAATAAGCACATCAGGCAGAG-3'

Other clones with same target sequence:

(none)