Construct: shRNA TRCN0000004557

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020774.x-1109s1c1
DNA Barcode:
GCCGAGTACAACAGATTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MIB1 (57534)
Gene Description:
mindbomb E3 ubiquitin protein ligase 1
Transcript:
RefSeq NM_020774.1 (NON-CURRENT)
Match location:
Position 1109 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004557)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAGTACAACAGATTTATT
Hairpin Sequence:
5'-CCGG-GCCGAGTACAACAGATTTATT-CTCGAG-AATAAATCTGTTGTACTCGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAGTACAACAGATTTATTCTCGAGAATAAATCTGTTGTACTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAGTACAACAGATTTATTCTCGAGAATAAATCTGTTGTACTCGGC-3'

Other clones with same target sequence:

  1. TRCN0000342502