Construct: shRNA TRCN0000004568

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020935.x-2591s1c1
DNA Barcode:
CCGGATTTGCAGAAGATGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
USP37 (57695)
Gene Description:
ubiquitin specific peptidase 37
Transcript:
RefSeq NM_020935.1 (NON-CURRENT)
Match location:
Position 2591 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004568)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGATTTGCAGAAGATGATA
Hairpin Sequence:
5'-CCGG-CCGGATTTGCAGAAGATGATA-CTCGAG-TATCATCTTCTGCAAATCCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGATTTGCAGAAGATGATACTCGAGTATCATCTTCTGCAAATCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGATTTGCAGAAGATGATACTCGAGTATCATCTTCTGCAAATCCGG-3'

Other clones with same target sequence:

(none)