Construct: shRNA TRCN0000004614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022750.x-819s1c1
DNA Barcode:
CCGGGAAGAACTGTAGGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PARP12 (64761)
Gene Description:
poly(ADP-ribose) polymerase family member 12
Transcript:
RefSeq NM_022750.1 (NON-CURRENT)
Match location:
Position 819 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGGAAGAACTGTAGGAATA
Hairpin Sequence:
5'-CCGG-CCGGGAAGAACTGTAGGAATA-CTCGAG-TATTCCTACAGTTCTTCCCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGGAAGAACTGTAGGAATACTCGAGTATTCCTACAGTTCTTCCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGGAAGAACTGTAGGAATACTCGAGTATTCCTACAGTTCTTCCCGG-3'

Other clones with same target sequence:

(none)