Construct: shRNA TRCN0000004633

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021332.1-887s1c1
DNA Barcode:
GCTGGACTAGGAACTCCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Glp1r (14652)
Gene Description:
glucagon-like peptide 1 receptor
Transcript:
RefSeq NM_021332.1 (NON-CURRENT)
Match location:
Position 887 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004633)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGACTAGGAACTCCAATA
Hairpin Sequence:
5'-CCGG-GCTGGACTAGGAACTCCAATA-CTCGAG-TATTGGAGTTCCTAGTCCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGACTAGGAACTCCAATACTCGAGTATTGGAGTTCCTAGTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGACTAGGAACTCCAATACTCGAGTATTGGAGTTCCTAGTCCAGC-3'

Other clones with same target sequence:

(none)