Construct: shRNA TRCN0000004663

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355385.1-1774s1c1
DNA Barcode:
GCATCTATACTGGCATCCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lgr4 (107515)
Gene Description:
leucine-rich repeat-containing G protein-coupled receptor 4
Transcript:
RefSeq XM_355385.1 (NON-CURRENT)
Match location:
Position 1774 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004663)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCTATACTGGCATCCTTA
Hairpin Sequence:
5'-CCGG-GCATCTATACTGGCATCCTTA-CTCGAG-TAAGGATGCCAGTATAGATGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCTATACTGGCATCCTTACTCGAGTAAGGATGCCAGTATAGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCTATACTGGCATCCTTACTCGAGTAAGGATGCCAGTATAGATGC-3'

Other clones with same target sequence:

(none)