Construct: shRNA TRCN0000004740

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018490.1-1563s1c1
DNA Barcode:
GCGTAATCAAATCTACCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LGR4 (55366)
Gene Description:
leucine rich repeat containing G protein-coupled receptor 4
Transcript:
RefSeq NM_018490.1 (NON-CURRENT)
Match location:
Position 1563 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004740)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTAATCAAATCTACCAAAT
Hairpin Sequence:
5'-CCGG-GCGTAATCAAATCTACCAAAT-CTCGAG-ATTTGGTAGATTTGATTACGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTAATCAAATCTACCAAATCTCGAGATTTGGTAGATTTGATTACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTAATCAAATCTACCAAATCTCGAGATTTGGTAGATTTGATTACGC-3'

Other clones with same target sequence:

  1. TRCN0000273532