Construct: shRNA TRCN0000004778

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130466.2-2982s1c1
DNA Barcode:
CGTGCCATTTGCATCCTTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBE3B (89910)
Gene Description:
ubiquitin protein ligase E3B
Transcript:
RefSeq NM_130466.2 (NON-CURRENT)
Match location:
Position 2982 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004778)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGCCATTTGCATCCTTCTT
Hairpin Sequence:
5'-CCGG-CGTGCCATTTGCATCCTTCTT-CTCGAG-AAGAAGGATGCAAATGGCACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGCCATTTGCATCCTTCTTCTCGAGAAGAAGGATGCAAATGGCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGCCATTTGCATCCTTCTTCTCGAGAAGAAGGATGCAAATGGCACG-3'

Other clones with same target sequence:

(none)