Construct: shRNA TRCN0000004790

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_038999.2-3820s1c1
DNA Barcode:
CCAGGGAAGTTAAAGTTAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HECW2 (57520)
Gene Description:
HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Transcript:
RefSeq XM_038999.2 (NON-CURRENT)
Match location:
Position 3820 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004790)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGGAAGTTAAAGTTAATT
Hairpin Sequence:
5'-CCGG-CCAGGGAAGTTAAAGTTAATT-CTCGAG-AATTAACTTTAACTTCCCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGGAAGTTAAAGTTAATTCTCGAGAATTAACTTTAACTTCCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGGAAGTTAAAGTTAATTCTCGAGAATTAACTTTAACTTCCCTGG-3'

Other clones with same target sequence:

(none)