Construct: shRNA TRCN0000004798

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024539.3-919s1c1
DNA Barcode:
GCTGTGTGCATTGAATTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF128 (79589)
Gene Description:
ring finger protein 128
Transcript:
RefSeq NM_024539.3 (CURRENT)
Match location:
Position 919 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004798)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGTGCATTGAATTGTAT
Hairpin Sequence:
5'-CCGG-GCTGTGTGCATTGAATTGTAT-CTCGAG-ATACAATTCAATGCACACAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGTGCATTGAATTGTATCTCGAGATACAATTCAATGCACACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGTGCATTGAATTGTATCTCGAGATACAATTCAATGCACACAGC-3'

Other clones with same target sequence:

(none)