Construct: shRNA TRCN0000004841

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006044.2-747s1c1
DNA Barcode:
GCACAGTCTTATGGATGGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HDAC6 (10013)
Gene Description:
histone deacetylase 6
Transcript:
RefSeq NM_006044.2 (NON-CURRENT)
Match location:
Position 747 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004841)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGTCTTATGGATGGCTA
Hairpin Sequence:
5'-CCGG-GCACAGTCTTATGGATGGCTA-CTCGAG-TAGCCATCCATAAGACTGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGTCTTATGGATGGCTACTCGAGTAGCCATCCATAAGACTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGTCTTATGGATGGCTACTCGAGTAGCCATCCATAAGACTGTGC-3'

Other clones with same target sequence:

(none)