Construct: shRNA TRCN0000004854

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014707.1-3334s1c1
DNA Barcode:
CCTAGAATCTTTGTGAGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HDAC9 (9734)
Gene Description:
histone deacetylase 9
Transcript:
RefSeq NM_014707.1 (NON-CURRENT)
Match location:
Position 3334 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004854)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGAATCTTTGTGAGGTTT
Hairpin Sequence:
5'-CCGG-CCTAGAATCTTTGTGAGGTTT-CTCGAG-AAACCTCACAAAGATTCTAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGAATCTTTGTGAGGTTTCTCGAGAAACCTCACAAAGATTCTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGAATCTTTGTGAGGTTTCTCGAGAAACCTCACAAAGATTCTAGG-3'

Other clones with same target sequence:

(none)