Construct: shRNA TRCN0000004892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003212.1-751s1c1
DNA Barcode:
GTCTGCACGTACTACCACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TDGF1 (6997)
Gene Description:
teratocarcinoma-derived growth factor 1
Transcript:
RefSeq NM_003212.1 (NON-CURRENT)
Match location:
Position 751 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTGCACGTACTACCACTTT
Hairpin Sequence:
5'-CCGG-GTCTGCACGTACTACCACTTT-CTCGAG-AAAGTGGTAGTACGTGCAGAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTGCACGTACTACCACTTTCTCGAGAAAGTGGTAGTACGTGCAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTGCACGTACTACCACTTTCTCGAGAAAGTGGTAGTACGTGCAGAC-3'

Other clones with same target sequence:

(none)