Construct: shRNA TRCN0000004912

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002542.4-2401s1c1
DNA Barcode:
CCTCCCTGTGACTACCTCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OGG1 (4968)
Gene Description:
8-oxoguanine DNA glycosylase
Transcript:
RefSeq NM_002542.4 (NON-CURRENT)
Match location:
Position 2401 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004912)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCCTGTGACTACCTCAAA
Hairpin Sequence:
5'-CCGG-CCTCCCTGTGACTACCTCAAA-CTCGAG-TTTGAGGTAGTCACAGGGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCCTGTGACTACCTCAAACTCGAGTTTGAGGTAGTCACAGGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCCTGTGACTACCTCAAACTCGAGTTTGAGGTAGTCACAGGGAGG-3'

Other clones with same target sequence:

(none)