Construct: shRNA TRCN0000004946

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016462.2-481s1c1
DNA Barcode:
CGCCAAAGTTGGAGTTAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM14C (51522)
Gene Description:
transmembrane protein 14C
Transcript:
RefSeq NM_016462.2 (NON-CURRENT)
Match location:
Position 481 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004946)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCAAAGTTGGAGTTAGTAT
Hairpin Sequence:
5'-CCGG-CGCCAAAGTTGGAGTTAGTAT-CTCGAG-ATACTAACTCCAACTTTGGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCAAAGTTGGAGTTAGTATCTCGAGATACTAACTCCAACTTTGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCAAAGTTGGAGTTAGTATCTCGAGATACTAACTCCAACTTTGGCG-3'

Other clones with same target sequence:

(none)