Construct: shRNA TRCN0000004977

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002114.1-8755s1c1
DNA Barcode:
CCAGGTAGTTATTTGTGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HIVEP1 (3096)
Gene Description:
HIVEP zinc finger 1
Transcript:
RefSeq NM_002114.1 (NON-CURRENT)
Match location:
Position 8755 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004977)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGTAGTTATTTGTGTTTA
Hairpin Sequence:
5'-CCGG-CCAGGTAGTTATTTGTGTTTA-CTCGAG-TAAACACAAATAACTACCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGTAGTTATTTGTGTTTACTCGAGTAAACACAAATAACTACCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGTAGTTATTTGTGTTTACTCGAGTAAACACAAATAACTACCTGG-3'

Other clones with same target sequence:

(none)