Construct: shRNA TRCN0000004983

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024953.2-2186s1c1
DNA Barcode:
CCGGATTGATATTCTTCGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAA25 (80018)
Gene Description:
N(alpha)-acetyltransferase 25, NatB auxiliary subunit
Transcript:
RefSeq NM_024953.2 (NON-CURRENT)
Match location:
Position 2186 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004983)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGATTGATATTCTTCGTTT
Hairpin Sequence:
5'-CCGG-CCGGATTGATATTCTTCGTTT-CTCGAG-AAACGAAGAATATCAATCCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGATTGATATTCTTCGTTTCTCGAGAAACGAAGAATATCAATCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGATTGATATTCTTCGTTTCTCGAGAAACGAAGAATATCAATCCGG-3'

Other clones with same target sequence:

(none)