Construct: shRNA TRCN0000005013

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006187.1-596s1c1
DNA Barcode:
CGGAGGAACTTTGTGAACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OAS3 (4940)
Gene Description:
2'-5'-oligoadenylate synthetase 3
Transcript:
RefSeq NM_006187.1 (NON-CURRENT)
Match location:
Position 596 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005013)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGGAACTTTGTGAACATT
Hairpin Sequence:
5'-CCGG-CGGAGGAACTTTGTGAACATT-CTCGAG-AATGTTCACAAAGTTCCTCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGGAACTTTGTGAACATTCTCGAGAATGTTCACAAAGTTCCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGGAACTTTGTGAACATTCTCGAGAATGTTCACAAAGTTCCTCCG-3'

Other clones with same target sequence:

  1. TRCN0000320370