Construct: shRNA TRCN0000005015

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006187.1-2078s1c1
DNA Barcode:
GCCATGAGAATGCACCTTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OAS3 (4940)
Gene Description:
2'-5'-oligoadenylate synthetase 3
Transcript:
RefSeq NM_006187.1 (NON-CURRENT)
Match location:
Position 2078 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005015)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGAGAATGCACCTTCTT
Hairpin Sequence:
5'-CCGG-GCCATGAGAATGCACCTTCTT-CTCGAG-AAGAAGGTGCATTCTCATGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGAGAATGCACCTTCTTCTCGAGAAGAAGGTGCATTCTCATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGAGAATGCACCTTCTTCTCGAGAAGAAGGTGCATTCTCATGGC-3'

Other clones with same target sequence:

(none)