Construct: shRNA TRCN0000005070

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016196.1-2012s1c1
DNA Barcode:
GCCTATTCCAAGTTCCATCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBM19 (9904)
Gene Description:
RNA binding motif protein 19
Transcript:
RefSeq NM_016196.1 (NON-CURRENT)
Match location:
Position 2012 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005070)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTATTCCAAGTTCCATCAT
Hairpin Sequence:
5'-CCGG-GCCTATTCCAAGTTCCATCAT-CTCGAG-ATGATGGAACTTGGAATAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTATTCCAAGTTCCATCATCTCGAGATGATGGAACTTGGAATAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTATTCCAAGTTCCATCATCTCGAGATGATGGAACTTGGAATAGGC-3'

Other clones with same target sequence:

  1. TRCN0000273200