Construct: shRNA TRCN0000005075

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000192.3-1638s1c1
DNA Barcode:
CCAAGAGGAAAGAGGAAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBX5 (6910)
Gene Description:
T-box transcription factor 5
Transcript:
RefSeq NM_000192.3 (CURRENT)
Match location:
Position 1638 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005075)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGAGGAAAGAGGAAGAAT
Hairpin Sequence:
5'-CCGG-CCAAGAGGAAAGAGGAAGAAT-CTCGAG-ATTCTTCCTCTTTCCTCTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGAGGAAAGAGGAAGAATCTCGAGATTCTTCCTCTTTCCTCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGAGGAAAGAGGAAGAATCTCGAGATTCTTCCTCTTTCCTCTTGG-3'

Other clones with same target sequence:

(none)